Regulatory

Part:BBa_J24671

Designed by: Azeem Kaka   Group: iGEM06_Brown   (2006-07-24)

Tri-Stable Toggle (Tetracycline induced component)

This component of the tri-stable toggle switch is activated by tetracycline and inhibits pLac and pAraBad by producing both LacI and AraC LVA-tagged proteins. This component is repressed by TetR. It is unterminated to allow for the addition of target genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None